A single generic microbore liquid chromatography/time-of-flight mass spectrometry solution for the simultaneous accurate mass determination of compounds on single beads, the decoding of dansylated orthogonal tags pertaining to compounds and accurate isotopic difference target analysis

Authors
Citation
Sj. Lane et A. Pipe, A single generic microbore liquid chromatography/time-of-flight mass spectrometry solution for the simultaneous accurate mass determination of compounds on single beads, the decoding of dansylated orthogonal tags pertaining to compounds and accurate isotopic difference target analysis, RAP C MASS, 13(9), 1999, pp. 798-814
Citations number
11
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
ISSN journal
09514198 → ACNP
Volume
13
Issue
9
Year of publication
1999
Pages
798 - 814
Database
ISI
SICI code
0951-4198(1999)13:9<798:ASGMLC>2.0.ZU;2-Z
Abstract
A single generic microbore liquid chromatography diode array/time-flight ma ss spectrometry method has been developed that provides accurate mass on se parated compounds cleaved from single beads and/or the decoding of orthogon al tags pertaining to the compound in one run. The method is suitable for e ncoded or non-encoded libraries. Accurate mass measurement within 5ppm can be routinely achieved allowing differentiation of isobaric compounds in deg enerate libraries. Accurate mass chromatograms can be used to resolve tag r esponses from polyethylene glycol background interference. Accurate isotope mass differences coupled with intensity ratios can be used for highly spec ific isotopic target analysis for compounds of unknown molecular weight but with a characteristic accurate isotope pattern. Accurate instrument calibr ation is not necessary with this approach to target analysis. Copyright (C) 1999 John Wiley & Sons, Ltd.