B. Kaiser et al., Identification of a gene encoding the pyruvate decarboxylase gene regulator CaPdc2p from Candida albicans, YEAST, 15(7), 1999, pp. 585-591
In a screen for Candida albicans genes encoding transactivating proteins, a
pyruvate decarboxylase (EC 4.1.1.1.) regulator gene was isolated. An open
reading frame (ORF) of 2511 bp was identified encoding a predicted protein
of 836 amino acids with a molecular weight of 94.4 kDa. The protein showed
glutamine- and proline-rich stretches typical for transcriptional activator
s. The amino acid sequence comparisons between CaPd2p of C. albicans and bo
th Pdc2p of Saccharomyces cerevisiae and Rag3p of Kluyveromyces lactis, rev
ealed similarities of 40% and 39%, respectively. The CaPDC2 gene was locali
zed on chromosome 1. Southern blot analysis indicated that CaPDC2 might be
a single copy gene. The growth defect of a S. cerevisiae pdc2 Delta mutant
on glucose was compensated by transformation of the C. albicans CnPDC2 gene
. The EMBL Accession No. for the sequence reported in this paper is Y17007.
Copyright (C) 1999 John Wiley & Sons, Ltd.