The fate of Yersinia pseudotuberculosis lipopolysaccharide (LPS) in intraperitoneally and intraarticularly infected rats

Persistence and in vivo effects of Yersinia pseudotuberculosis serotype III LPS (prepared from bacteria grown at 25 degrees and 37 degrees C) in rats were investigated after intraperitoneal and intraarticular injection during the 30 day period of examination. Localization and persistence of LPS in t he peritoneal and synovial cavities were demonstrated by using the immunofl uorescence technique. Peritoneal and synovial exudative cell infiltration a s well as changes in some parameters (glycolytic and acid phosphatase activ ities, killing ability of peritoneal cells, lactate-dehydrogenase concentra tion in synovial fluid) were studied. The results indicated that LPS expres sed at 37 degrees C induced stronger peritoneal cell response (increased "k illing" ability and elevation of glycolytic and acid phosphatase activities ) in comparison to that synthesized at 25 degrees C. It was also found, tha t LPS persisted longer in the synovial rather than in the peritoneal cavity of rats and induced long-lasting synovial inflammation.