Role of the human endometrial coculture system on murine embryo development exposed to human hydrosalpinx fluid

Several studies suggest that hydrosalpinx fluid (HF) exerts a deleterious e ffect on the in vitro murine embryo development in a dose-dependent manner, these studies were performed evaluating blastocyst stage on a simple cultu re. The present investigation was to assess whether an endometrial co-cultu re system protects the effect of HF on murine development. We evaluated the blastocyst cell number in a simple (SCS) and endometrial co-culture system (ECS). When embryos were exposed to 50% and 70% of HF a significant diminu tion in the blastocyst cell number with respect to the control was observed in co-culture. Similar results on the embryo development were seen in simp le culture. Thus, our results suggest that HF exert a deleterious effect on murine embryos despite the culture system employed.