Crystallization and preliminary diffraction analysis of Escherichia coli cysteinyl-tRNA synthetase

Authors
Newberry, KJ Kohn, J Hou, YM Perona, JJ
Citation
Kj. Newberry et al., Crystallization and preliminary diffraction analysis of Escherichia coli cysteinyl-tRNA synthetase, ACT CRYST D, 55, 1999, pp. 1046-1047
Citations number
18
Categorie Soggetti
Chemistry & Analysis
Journal title
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
ISSN journal
09074449 → ACNP
Volume
55
Year of publication
1999
Part
5
Pages
1046 - 1047
Database
ISI
SICI code
0907-4449(199905)55:<1046:CAPDAO>2.0.ZU;2-3
Abstract
Crystals of the 52 kDa monomeric Escherichia coli cysteinyl-tRNA synthetase complexed with ATP and cysteine have been grown by hanging-drop vapor diff usion from solutions containing ammonium sulfate as the precipitating agent . The crystals form long hexagonal rods in the space group P321 with unit-c ell dimensions a = b = 82.3, c = 168.9 Angstrom. There is one enzyme molecu le in the asymmetric unit. A complete native data set has been collected fr om a rotating-anode source to a resolution of 2.7 Angstrom at 103 K, with a n R-merge of 6.7%.