Crystallization of a precursor penicillin acylase from Escherichia coli

Authors
Hewitt, L Kasche, V Lummer, K Rieks, A Wilson, KS
Citation
L. Hewitt et al., Crystallization of a precursor penicillin acylase from Escherichia coli, ACT CRYST D, 55, 1999, pp. 1052-1054
Citations number
12
Categorie Soggetti
Chemistry & Analysis
Journal title
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
ISSN journal
09074449 → ACNP
Volume
55
Year of publication
1999
Part
5
Pages
1052 - 1054
Database
ISI
SICI code
0907-4449(199905)55:<1052:COAPPA>2.0.ZU;2-5
Abstract
The crystallization of a mutant precursor penicillin acylase [penicillin am idohydrolase (amidase) E.C 3.5.1.11] from Escherichia coli W (ATCC 11105) u sing the hanging-drop method is reported. The crystals are in space group P 1 with unit-cell parameters a = 51.04, b = 63.58, c = 71.17 Angstrom, alpha = 103.0, beta = 110.6, gamma = 105.3 degrees, with one molecule in the asy mmetric unit, and diffract to 1.8 Angstrom using synchrotron radiation.