Crystallization and preliminary X-ray analysis of chondroitinase B from Flavobacterium heparinum

Chondroitinase B, a glycosaminoglycan lyase from Flavobacterium heparinum, has been crystallized by hanging-drop vapor diffusion in space group P2(1) with unit-cell parameters a = 50.6, b = 74.5, c= 58.7 Angstrom, beta = 92.9 degrees and one molecule in the asymmetric unit. This enzyme degrades derm atan sulfate, a glycosaminoglycan primarily made up of a disaccharide repea ting unit of iduronic acid and N-acetylgalactosamine, A complete native dat a set has been collected from a single crystal to 2.2 Angstrom resolution u sing a rotating-anode source.