Crystallization and preliminary X-ray crystallographic analysis of a Trichoderma reesei beta-mannanase from glycoside hydrolase family 5

Crystals of the catalytic core domain of a Trichoderma reesei beta-mannanas e belonging to glycoside hydrolase family 5 have been grown by the sitting- drop method at room temperature using ammonium sulfate as precipitant. The crystals grow as thin colourless plates and belong to space group P2(1), wi th unit-cell parameters a = 50.0, b = 54.3, c = 60.2 Angstrom, beta = 111.3 degrees, and have a single monomer of mannanase in the asymmetric unit. Na tive data to 2.0 Angstrom resolution have been collected at room temperatur e using synchrotron radiation. Data for a platinum derivative have been col lected to 1.65 Angstrom at 110 K in a very short time at the CCLRC Daresbur y synchrotron source, using a charge-coupled device (CCD) as detector.