Crystallization and preliminary X-ray crystallographic analysis of deoxycytidylate hydroxymethylase from bacteriophage T4

Deoxycytidylate hydroxymethylase from bacteriophage T4 is a homodimeric enz yme in which each polypeptide chain consists of 246 amino-acid residues. It has been crystallized in the presence of its substrate, deoxycytidine mono phosphate, at room temperature using sodium citrate as precipitant. The cry stals are monoclinic, belonging to space group C2, with unit-cell parameter s a = 174.22, b = 53.12, c = 75.17 Angstrom, beta = 115.29 degrees. The asy mmetric unit contains one homodimer, with a corresponding V-m of 2.65 Angst rom(3) Da(-1) and solvent content of 54%. Native diffraction data to 1.6 An gstrom resolution have been collected from two crystals using synchrotron r adiation.