Cell injury and interstitial inflammation in rat lung after inhalation of ozone and urban particulates

Citation
Iyr. Adamson et al., Cell injury and interstitial inflammation in rat lung after inhalation of ozone and urban particulates, AM J RESP C, 20(5), 1999, pp. 1067-1072
Citations number
21
Categorie Soggetti
da verificare
Journal title
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
ISSN journal
10441549 → ACNP
Volume
20
Issue
5
Year of publication
1999
Pages
1067 - 1072
Database
ISI
SICI code
1044-1549(199905)20:5<1067:CIAIII>2.0.ZU;2-U
Abstract
Coexposure of the lung to urban dust along with ozone appears to potentiate ozone-induced injury. This conclusion was derived from whole-lung studies involving tissue and lavaged cells, but we now speculate that the injury an d inflammatory response at the main site of reactivity, the bronchoalveolar duct region, is underestimated by such whole-lung studies. We exposed rats to ozone at 0.8 ppm and urban particulates (EHC93) at 50 mg/m(3) for 4 h. Animals were killed 33 h later with tritiated thymidine ((HT)-H-3) injected 1.5 h before death. Lungs were fixed by vascular perfusion;to avoid distur bing any epithelial cell changes or local inflammation and edema in the air spaces. Tissue was embedded from central and peripheral areas of the lung, then counts of labeled cells, polymorphonuclear leukocytes (PMN), and macr ophages (MAC) were made separately on methacrylate sections. The results sh owed that epithelial cell injury and regeneration (% of (HT)-H-3-labeled ce lls) was greatest in the ozone plus dust group, and was three times higher in periductal areas than in whole-lung counts. Although some increase in in flammatory cells in the air spaces was found in the coexposure group, much higher numbers of PMN and MAC were counted in the lung tissue compartment, and counts were significantly higher than those found after ozone or dust a lone. Values from the latter groups were also higher than those from air co ntrols or samples of distal lung taken from any inhalation group. The resul ts demonstrate that inhalation of an urban dust at a level that causes few lung effects when inhaled alone can potentiate ozone toxicity, particularly in the vicinity of the alveolar duct, where the accumulation of interstiti al inflammatory cells may be an important factor in the development of any subsequent pathologic changes.