J. Saurina et S. Hernandez-cassou, A comparison of chemometric methods for the flow injection simultaneous spectrophotometric determination of aniline and cyclohexylamine, ANALYST, 124(5), 1999, pp. 745-749
A flow injection spectrophotometric method for the simultaneous determinati
on of aniline and cyclohexylamine using multivariate calibration methods is
proposed. The method is based on the reaction of these amines with 1,2-nap
hthoquinone-4-sulfonate, yielding spectrophotometrically active derivatives
. The data analysed with multivariate calibration methods consisted of the
spectra registered in the range 290-590 nm at the maximum of the flow injec
tion peak. Although the spectrum of each derivative was characteristic, ove
rlapping occurred and no selective wavelengths were found. The predictive a
bilities of principal component regression and partial least-squares regres
sion (PLS), non-linear PLS, locally weighted regression(LWR) and artificial
neural networks were examined for the determination of aniline and cyclohe
xylamine in sample mixtures. The accuracy for cyclohexylamine and aniline q
uantifications in unknown mixtures was optimum with LWR, providing overall
prediction errors of 3.4 and 5.6%, respectively.