Jc. Yuan et Am. Shiller, Determination of subnanomolar levels of hydrogen peroxide in seawater by reagent-injection chemiluminescence detection, ANALYT CHEM, 71(10), 1999, pp. 1975-1980
A reagent-injection chemiluminescent method for the determination of hydrog
en peroxide (H2O2) has been developed. The method is based on the catalytic
(cobalt (IT)) oxidation of luminol by hydrogen peroxide in an alkaline sol
ution. One mixed reagent is used for the analysis using the reagent injecti
on method. The mixed reagent is optimized for pH and concentration of lumin
ol and Co2+. Apart from Fe2+, none of the 14 species tested showed interfer
ence at their seawater levels. Because Fe2+ oxidation is rapid compared to
the rate of decay of hydrogen peroxide, the Fe2+ interference can be elimin
ated by storing seawater samples for 1 h. The combination of reagents and t
he adaptation of reagent injection lead to a subnanomolar detection limit.
The accuracy of the method is at least 0.42 nM. The precision of the method
is 17 pM (for a 0.57 nM sample). The method has been used aboard ship to d
etermine the concentrations of hydrogen peroxide in seawater samples in the
open ocean as well as on the continental shelf.