K. Ikeda et al., Involvement of hydrogen peroxide and hydroxyl radical in chemically induced apoptosis of HL-60 cells, BIOCH PHARM, 57(12), 1999, pp. 1361-1365
Effects of three kinds of antagonists against reactive oxygen species were
evaluated at the same time in chemically induced apoptosis of human leukemi
c HL-60 cells. Apoptosis of HL-60 cells induced by actinomycin D, H7, 1-bet
a-D-arabinofuranosylcytosine, and daunorubicin was inhibited significantly
by radical scavengers (vitamin E, N-acetyl-L-cysteine, and mercaptoethanol)
, catalase, and a spin trap, N-t-butyl-alpha-phenylnitrone. These results s
uggest that hydrogen peroxide and hydroxyl radical are common mediators of
apoptosis caused by these chemicals with apparently different functional me
chanisms. The consumption of vitamin E to inhibit apoptosis induced by acti
nomycin D was undetectable, suggesting that the generation of reactive oxyg
en species during apoptosis was not very extensive. Radicals were suggested
to be a mediator of apoptosis of HL-60 cells induced by cisplatin based on
thr observations that the above inhibitors, except catalase, effectively i
nhibited apoptosis by the drug. (C) 1999 Elsevier Science Inc.