Enhancing solid phase synthesis by a noncovalent protection strategy-efficient coupling of rhodamine to resin-bound peptide nucleic acids

Resins for solid-phase synthesis can affect coupling efficiencies by intera cting with reactants. We have observed that polyethylene glycol-polystyrene (PEG-PS) solid support absorbs added activated fluorophores, preventing ef ficient labeling of peptide nucleic acids (PNAs). We now report that additi on of an inexpensive unactivated fluorophore blocks the resin and allows ef ficient labeling. This protection strategy may have general benefits for pe ptide and combinatorial synthesis, (C) 1999 Elsevier Science Ltd. All right s reserved.