Intraperitoneal gene therapy with adenoviral-mediated p53 tumor suppressorgene for ovarian cancer model in nude mouse

In an effort to develop a method for better local control of advanced ovari an cancers, we have established a peritoneal tumor model of ovarian cancer in the nude mouse and applied intraperitoneal gene therapy with the recomin ant adenoviral-mediated wild-type p53 tumor suppressor gene (Avp53). The re sults indicate that: (a) the recombinant adenoviral vector system effective ly infected the tumor and normal cells in the peritoneal cavity; and (b) Av p53 treatment effectively suppressed the growth of peritoneal tumors and pr olonged the survival of the treated group, especially when the tumor burden was less. These results suggest that intraperitoneal gene therapy using Av p53 is potentially useful as an adjuvant therapeutic modality in human ovar ian cancer.