Human erythrocyte acetylcholinesterase inhibition by cis-diamminediaquaplatinum (II): a novel kinetic approach

The present work addresses the analyses of some novel kinetic parameters (k (t), K-v, t(50), K-ir, t(c), m(c), IC50, IC99 and K-i) of human erythrocyte membrane-bound acetylcholinesterase (AChE, EC 3.1.1.7) inhibition by cis-d iamminediaquaplatinum II (PDC). PDC is under a clinical trial for use as an antineoplastic drug. The authors recently reported that PDC and cisplatin have the ability to inhibit AChE activity in vitro. Therefore this study wa s designed to determine the estimation of time constant (k(t)), velocity co nstant (K-v), 50% inhibition time (t(50)), inhibition rate constant (K-ir), transition concentration (t(c)), meeting concentration (m(c)), 50% inhibit ion (IC50), 99% inhibition (IC99) and inhibition constant (K-i) by novel me thods. The details are described in the text. (C) 1999 Elsevier Science Ire land Ltd. All rights reserved.