Motility inhibition and apoptosis are induced by metastasis-suppressing gene product CD82 and its analogue CD9, with concurrent glycosylation

Metastasis-suppressing gene product CD82 and its analogue CD9 are considere d to suppress the malignancy of various human cancers, although the rationa le for this effect is unknown. The present study addresses phenotypic chang es in Chinese hamster ovary mutant cell line ldlD deficient in UDP-Glc 4-ep imerase and expressing CD82 or CD9 by cDNA transfection. Only CD82- or CD9- expressing cells grown in Gal-supplemented medium showed reduced motility a nd massive cell death, which are characteristic of apoptosis, after a laten t period. Under this condition, endogenous GM3 synthesis was observed as a common factor, and N-glycosylation occurred at a high level in CD82 and to a lesser extent in CD9, Thus, the malignancy-suppressing effect of CD82 or CD9 is based partially on cell motility inhibition and apoptosis induction promoted by concurrent GM3 synthesis and N-glycosylation.