K. Mizuishi et al., DIRECT GC DETERMINATION OF METHYLMERCURY CHLORIDE ON HBR-METHANOL-TREATED CAPILLARY COLUMNS, Chromatographia, 44(7-8), 1997, pp. 386-392
Citations number
25
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Gas chromatography with electron capture detection (GC-ECD) for the an
alysis of methylmercury chloride (MMC) using a packed column and a cap
illary column has been investigated. The columns were 2 % silicone OV-
227 Uniport HP glass column and a DB-17 capillary column, each pretrea
ted by about ten injections of HBr-methanol solution. MMC was separate
d as a sharp peak by the HBr-teated column and determined directly by
ECD without derivatisation. The mass spectrum of MMC indicated that ha
lide exchange from chloride to bromide proceeded during separation. Th
e minimum detectable concentrations were approximately 5 ng mL(-1) on
the packed column, and 2 ng mL(-1) on the capillary. Calibration curve
s showed good linearity between 5-200 ng mL(-1) for the packed column,
and between 2-200 ng mL(-1) for the capillary. Relative standard devi
ations of peak areas were 0.95 % for the packed column and 0.43 % for
the capillary at the level of 100 ng mL(-1) in both cases. The column
treatment technique was applicable to determination of methylmercury i
n fish samples.