Preliminary characterization of a reovirus isolated from golden ide Leuciscus idus melanotus

Some characteristics of a reovirus recently isolated from golden ide Leucis cus idus melanotus and tentatively designated as golden ide reovirus (GIRV) were determined. Spherical nonenveloped particles with an outer capsid of about 70 nm and an inner capsid of about 50 nm were observed by electron mi croscopy. The density of the virus determined in CsCl gradients was 1.36 g ml(-1). The genome contained 11 segments of dsRNA. GIRV differed from other aquareoviruses by a slight reduction of infectivity after treatment with c hloroform and by the absence of forming syncytia in cell monolayers.