A microtiter-based assay was developed to study the binding of Helicobacter
pylori to pig gastric mucins purified by density-gradient centrifugation i
n CsCl/4 M guanidinium chloride. Binding of H. pylori was observed over the
'mucin' band as well as with 'low-density' components in the gradients, an
d binding to the latter was more pronounced when incubations were performed
at 37 degrees C as compared to 20 degrees C. At a lower pH, binding of H.
pylori (strain SVA 40) to the 'high-density' mucins from pig antrum was inc
reased but binding to the 'low-density' ones was decreased. Binding of the
P466 strain (Le(b)-specific) was mainly associated with the 'mucin' band, w
hereas the MO19 strain reacted preferentially with the 'low-density' compon
ents. In summary, H. pylori may bind to gastric mucins and the binding is i
nfluenced by temperature, pH and the repertoire of bacterial adhesins. (C)
1999 Federation of European Microbiological Societies. Published by Elsevie
r Science B.V. All rights reserved.