FreeTree-freeware program for construction of phylogenetic trees on the basis of distance data and bootstrap jackknife analysis of the tree robustness. Application in the RAPD analysis of genus Frenkelia.

The Win95 program for computation of distance matrixes and construction of phylogenetic or phenetic trees on the basis of RAPD, RFLP and allozyme data was presented. In contrast with other presently available software, the pr ogram FreeTree can also assess the robustness of the tree topology by boots trap, jackknife or OTU-jackknife analysis. Moreover, the program can be use d also for an analysis of data obtained in several independent experiments performed with nonidentical subsets of taxa. The function of the program wa s demonstrated by an analysis of RAPD data from 22 strains of Frenkelia. Th e program is available as an autoextractive archive containing the installa tion files of FreeTree and TreeView, manual in MS Word format and a sample of the input file at http ://www.natur,cuni. cz/similar to flegr/programs/f reetree.