A contribution to the improvement of accuracy in the quantitation of THC

The accuracy of a quantitative analysis is highly dependent on the quality of the reference standard. Although reference standards are more and more s upplied with a certificate, laboratories may feel the need for additional a cceptance testing. In general, confirmation of the purity of many solid ref erence substances can be obtained by a number of simple tests. However, ver ification of the true content of reference solutions may be complicated. A number of problems with the THC quantitation caused our interest for a veri fication method for the THC reference solution. The quantitation of THC is performed by gas chromatography with flame ionisation detector. The effecti ve carbon number concept was used to predict GC/FID response factors. Equat ions and data are presented to calculate theoretical response ratios of can nabinoids. The experimental data for CBD and CBN were in excellent agreemen t with the theoretical ones, The paper shows that the response factors of C BD and/or CBN can be used for the calculation of the THC content of either reference solutions or cannabis samples. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.