We describe developments of regression-based methods for estimating the rel
ationship between gene flow and distance using allelic variation at marker
loci. These new methods allow the correct testing of the difference between
regression slopes, and the assignment of confidence intervals to estimates
from the regression equation, when the regression is between matrices of p
airwise data, rather than between independent values. We show that there ar
e significant differences in gene flow estimates from RFLPs and isozymes in
sea beet. Estimates of the distance at which populations exchange one migr
ant per generation are lower for wild cabbage than for sea beet.