Introduction of multi-alien chromatins carrying different powdery mildew-resistant genes from rye and Haynaldia villosa into wheat genome

Powdery mildew-resistant wheat lines, Tra.R9002 with rye chromatin and Tra. V149 with Haynaldia villosa chromatin, were crossed to combine their differ ent powdery mildew-resistant genes in the wheat genetic background. In the F-5 generation of Tra.R9002 x Tra.V149, a highly powdery mildew-resistant l ine W159 was selected according to its chromosome number 2n = 41 similar to 42. In the Fs line W159-9 which was immune to powdery mildew one pair of r ye chromosomes and one pair of wheat-H. villosa translocated chromosomes we re identified simultaneously by multicolor fluorescence genomic: in situ hy bridization in the wheat genetic background (chromosome number 2n = 42). Th rough C-banding and a sequential C-banding analysis after genomic in situ h ybridization, the rye chromosomes were identified as 5R and the wheat-H. vi llosa translocated chromosomes were identified as 6VS-6AL. This indicates t hat W159-9 carries two powdery mildew-resistant genes of Pm4 on 5R and Pm21 on 6VS. The wheat chromosomes absent in W159-9 were identified as the 6AS and 5D. On the other hand, seven out of 17 progenies of another F-6 line W1 59-3 possessed 5R and 6VS-6AL chromosomes. These materials could be used fo r acquiring stable multiple powdery mildew-resistance in wheat breeding pro grams.