An automated sample preparation system for large-scale DNA sequencing

Recent advances in DNA sequencing technologies, both in the form of high la ne-density gels and automated capillary systems, will lead to an increased requirement for sample preparation systems that operate at low cost and hig h throughput. As part of the development of a fully automated sequencing sy stem, we have developed an automated subsystem capable of producing 10,000 sequence-ready ssDNA templates per day From libraries of M13 plaques at a c ost of $0.29 per sample. This Front End has been in high throughput operati on since June, 1997 and has produced > 400,000 high-quality DNA templates.