A radiation hybrid map of mouse chromosome 13

A mouse radiation hybrid (RH) panel was used to make a framework map for th e entire length of mouse chromosome (Chr) 13. Forty-one loci were typed, an d while most used primers flanking simple sequence repeats, some genes were included. The most proximal and distal loci are D13Mit132 and D13Mit35. Th e estimate of map length for Chr 13 is 1328 cR. The map is compared with th e same set of loci from the consensus map for Chr 13, which is 70 cM in len gth, and also with a recombinational map derived from an intraspecies cross typed for many of the same loci. The mouse RH panel gave good resolution f or Chr 13 and at the distal end allowed separation of previously nonrecombi nant markers that are present on a single 620-kb YAC clone. Data analysis w as performed using the RH option for Map Manager QT. This framework RH map of Chr 13 is the second of a series of RH maps for mouse chromosomes. (C) 1 999 Academic Press.