My. Lim et al., Enhanced dissolution of gallstone by combining ethanol with two commonly used cholelitholytic solvents, HEP-GASTRO, 46(26), 1999, pp. 758-761
BACKGROUND/AIMS: Contact dissolution therapy is one of the non-surgical tre
atments for patients with gallstone. Among the various solvents, methyl ter
t-butyl ether (MTBE) is used for cholesterol gallstone, while tetrasodium e
thyl-dimethyl tetraacetate (EDTA-4Na) solution is used to dissolve calcium
bilirubinate stones. However, the contents of gallstone cannot be precisely
predicted while they are still present in the human body. This study was d
esigned to test if the MTBE and EDTA can be mixed together and to test the
solubility of different kinds of gallstone in each original solution and mi
xture.
METHODOLOGY: Each 0.1gm of mixed cholesterol stone, brown stone and pigment
stone from 18 patients was used. Pure ethanol was chosen to enhance the mi
scibility between the organic phase of MTBE and the aqueous phase of EDTA.
The contents of gallstone after dissolution were examined with scanning ele
ctromicroscopy.
RESULTS: We found the mixture of ethanol, MTBE and EDTA to be the most effi
cient solvent in gallstone dissolution in comparison with the other two ori
ginal solvents. The mixture reached a dissolution percentage of 97.96+/-1.0
0, 88.96+/-6.51 and 67.75 +/- 14.26 for cholesterol, brown and black pigmen
t gallstone, respectively.
CONCLUSIONS: We concluded that ethanol can be used to mix the MTBE and EDTA
with good preservation in their litholytic effects on gallstone. The ethan
ol-MTBE-EDTA solvent is,therefore, a promising universal cholelitholytic ag
ent which deserves further tests for its safety and efficacy in the in vivo
study.