Coinfection of Spodoptera exigua and Spodoptera frugiperda cell lines withthe nuclear polyhedrosis viruses of Autographa californica and Spodoptera exigua
T. Yanase et al., Coinfection of Spodoptera exigua and Spodoptera frugiperda cell lines withthe nuclear polyhedrosis viruses of Autographa californica and Spodoptera exigua, INTERVIROLO, 41(6), 1998, pp. 244-252
The Autographa californica multinucleocapsid nuclear polyhedrosis virus (Ac
MNPV) has a broad host range among Lepidoptera, In contrast, the Spodoptera
exigua MNPV (SeMNPV) can replicate efficiently only in S. exigua larvae or
S. exigua-derived cell lines. In this study, we examined the coinfection o
f S. exigua Se301 and Spodoptera frugiperda IPLB-SF21AEII (Sf21) cell lines
with SeMNPV and AcMNPV recombinant (Ac360-501 beta-gal) which was construc
ted for expression of P-galactosidase under control of the polyhedrin promo
ter. Coinfection led to the restriction as the level of late gene expressio
n, nonoccluded virus production, and DNA replication of Ac360-501 beta-gal
in both Se301 and Sf21 cell lines. In contrast, Ac360-501p-gnE supported th
e SeMNPV replication in Sf21 cells. Occurrence of recombinants, between Ac3
60-501 beta-gal and SeMNPV, with expanded host range was not observed in co
infected Sf21 cells. This suggests that Ac360-501 beta-gal supports the SeM
NPV replication through trans-activation.