Functional characterization of bovine parainfluenza virus type 3 hemagglutinin-neuraminidase and fusion proteins expressed by adenovirus recombinants

We constructed replication-competent human adenovirus type 5 (HAd5) recombi nants (HAd5-HN and HAd5-F) containing the bovine parainfluenza virus type 3 (BPIV3) hemagglutinin-neuraminidase (HN) or fusion (F) gene under the cont rol of the simian virus 40 (SV40) regulatory sequences. These genes were in serted in the early region 3 (E3) of the HAd5 genome in the E3 parallel ori entation. Expression of HN or F in HAd5-HN- or HAd5-F-infected cell extract s, respectively, was observed by immunoprecipitation using a BPIV3-specific polyclonal antiserum, Our results suggest that HN and F expressed by HAd5 recombinants were functionally similar to the native HN and F expressed in BPIV3-infected cells.