A. Bebenek et al., Bacteriophage T4 rnh (RNase H) null mutations: Effects on spontaneous mutation and epistatic interaction with rII mutations, J BACT, 181(10), 1999, pp. 3123-3128
The bacteriophage T4 mh gene encodes T4 RNase H, a relative of a family of
flap endonucleases. T4 mh null mutations reduce burst sizes, increase sensi
tivity to DNA damage, and increase the frequency of acriflavin resistance (
Ac-r) mutations. Because mutations in the related Saccharomyces cerevisiae
RAD27 gene display a remarkable duplication mutator phenotype, we further e
xplored the impact of mh mutations upon the mutation process. We observed t
hat most Ac-r mutants in an rnh(+) strain contain ac mutations, whereas onl
y roughly half of the Ac-r mutants detected in an rnh Delta strain bear ac
mutations. In contrast to the mutational specificity displayed by most muta
tors, the DNA alterations of ac mutations arising in rnh Delta and rnh(+) b
ackgrounds are indistinguishable. Thus, the increase in Ac-r mutants in an
rnh Delta background is probably not due to a mutator effect. This conclusi
on is supported by the lack of increase in the frequency of rI mutations in
an rnh Delta background. In a screen that detects mutations at both the rI
locus and the much larger rII locus, the r frequency was severalfold lower
in an rnh Delta background. This decrease was due to the phenotype of mh r
II double mutants, which display an r(+) plaque morphology but retain the c
haracteristic inability of rII mutants to grow on lambda lysogens. Finally,
we summarize those aspects of T4 forward-mutation systems which are releva
nt to optimal choices for investigating quantitative and qualitative aspect
s of the mutation process.