Regulation of beta-galactosidase expression in Bacillus megaterium DSM319 by a Xy1S/AraC-Type transcriptional activator

Authors
Strey, J Wittchen, KD Meinhardt, F
Citation
J. Strey et al., Regulation of beta-galactosidase expression in Bacillus megaterium DSM319 by a Xy1S/AraC-Type transcriptional activator, J BACT, 181(10), 1999, pp. 3288-3292
Citations number
32
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF BACTERIOLOGY
ISSN journal
00219193 → ACNP
Volume
181
Issue
10
Year of publication
1999
Pages
3288 - 3292
Database
ISI
SICI code
0021-9193(199905)181:10<3288:ROBEIB>2.0.ZU;2-Z
Abstract
The beta-galactosidase-encoding bgaM gene of Bacillus megaterium DSM319 and the divergently orientated bgaR operon were isolated and sequenced. Both t raits are subject to catabolite repression. A set of single-gene replacemen t mutants was generated and used to analyze gene function. BgaR was found t o be a XylS/AraC-type positive transcriptional regulator of bgaM; a potenti al regulator binding site overlaps the bgaM promoter. A mechanism for regul ation of beta-galactosidase expression in B. megaterium is proposed.