Expression of N-15-labeled eukaryotic cytochrome c in Escherichia coli

Authors
Morar, AS Kakouras, D Young, GB Boyd, J Pielak, GJ
Citation
As. Morar et al., Expression of N-15-labeled eukaryotic cytochrome c in Escherichia coli, J BIOL I CH, 4(2), 1999, pp. 220-222
Citations number
22
Categorie Soggetti
Chemistry & Analysis
Journal title
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
ISSN journal
09498257 → ACNP
Volume
4
Issue
2
Year of publication
1999
Pages
220 - 222
Database
ISI
SICI code
0949-8257(199904)4:2<220:EONECC>2.0.ZU;2-S
Abstract
We present a simple and inexpensive method for producing N-15-labeled Sacch aromyces cerevisiae iso-1-cytochrome c in Escherichia coli. The labeled pro tein gives excellent NMR spectra.