One-step reverse transcriptase PCR method for detection of Borrelia burgdorferi mRNA in mouse Lyme arthritis tissue samples

Authors
Limbach, FX Jaulhac, B Piemont, Y Kuntz, JL Monteil, H Sibilia, J
Citation
Fx. Limbach et al., One-step reverse transcriptase PCR method for detection of Borrelia burgdorferi mRNA in mouse Lyme arthritis tissue samples, J CLIN MICR, 37(6), 1999, pp. 2037-2039
Citations number
15
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
00951137 → ACNP
Volume
37
Issue
6
Year of publication
1999
Pages
2037 - 2039
Database
ISI
SICI code
0095-1137(199906)37:6<2037:ORTPMF>2.0.ZU;2-G
Abstract
A one-step reverse transcriptase PCR (RT-PCR) method for detection of Borre lia burgdorferi mRNA in infected C3H mice is described. This simple procedu re, less prone to nucleic acid cross-contamination than the standard method , was found to be 10-fold more sensitive than a classical two-step RT-PCR a ssay. By using onestep RT-PCR, flagellin mRNAs were detected in synovial an d heart tissues from all seven infected mice tested.