Consumption of microalgae by the grass shrimp Palaemonetes pugio

The ability of Palaemonetes pugio to consume microalgae in marshes of Spart ina alterniflora was examined in field collections and laboratory experimen ts. Photosynthetic pigment (chlorophyll a and phaeopigments) content in P. pugio was measured after shrimp exoskeletons were removed to ensure that pi gments were primarily from gut contents. Gut pigment was detectable in all 88 field-collected specimens examined, and was found at high levels in many individuals (much higher than shrimp starved for 24 h). Pigment content wa s positively correlated with shrimp size and was highly variable. Adults co ntained from 0.056-3.03 and subadults from 0.016-2.41 mu g pigment shrimp(- 1), 14% of which was in the form of chlorophyll a. Highest pigment levels w ere associated with adult grass shrimp collected at 1500 h; pigment content at 0300 h was significantly lower. The ability of P. pugio to consume micr oalgae from various sources was examined in the laboratory with C-14-labele d microalgae and gut-pigment analysis. Palaemonetes pugio consumed two spec ies of cultured, C-14-labeled phytoplanktan, but with low efficiency at eve n very high concentrations. For Thalassiosira weissflogii, 2.5%, and for Is ochrysis galabana, 0.07% of the available cells were consumed in 1-h trials at high concentrations of algae. Unvegetated, mudflat sediment (with a hig h abundance of benthic algae) was collected and labeled with C-14 bicarbona te. Incorporation of label above background was detected in a low percentag e of shrimp, and average grazing rates were very low (near detection limits ), suggesting that grass shrimp are not efficient grazers of the microphyto benthos. Spartina alterniflora stems with C-14-labeled epiphytic algae were also offered to grass shrimp. Grazing was highly efficient (a substantial fraction of the available epiphytic algae was consumed), and pigment conten t was similar to held-collected shrimp. These results suggest that microalg ae may significantly contribute to the diet of grass shrimp, and that a lik ely source for the photosynthetic pigments in the gut contents of held-coll ected P: pugio is the epiphytic algae on stems of Spartina alterniflora.