Sepsis increases intracellular free calcium in brain

Using magnetic resonance methods and a clinically relevant rodent model of sepsis, we have made in vivo measurements of increased intracellular calciu m in a pathologic state in the CNS. The intracellular calcium concentration was increased nearly twofold in septic rat brain compared with controls (p < 0.0001). This result, in a fully intact functioning mammalian system, ti es together a previous spectrum of indirect evidence from numerous laborato ries suggesting an important role for elevated intracellular calcium in sep sis. In addition, levels of the proinflammatory cytokine tumor necrosis fac tor-ct were elevated threefold in septic rat brain (p < 0.02), and electron microscopic examination revealed scattered injury in similar to 0.25% of g lial cells. These findings are discussed in light of the current understand ing of the pathophysiology of sepsis.