Dc. Widenka et al., Inducible nitric oxide synthase: a possible key factor in the pathogenesisof chronic vasospasm after experimental subarachnoid hemorrhage, J NEUROSURG, 90(6), 1999, pp. 1098-1104
Object. The role of nitric oxide (NO) in the pathogenesis of cerebral vasos
pasm after subarachnoid hemorrhage (SAH) is not well understood, Nitric oxi
de is a well-established vasodilatory substance: however, in SAH, NO may be
come a major source for the production of injurious free-radical species, l
ending to chronic cerebral vasospasm, Reactive overproduction of NO to coun
teract vascular narrowing might potentiate the detrimental effects of NO. T
he focus of the present study is to determine the extent of reactive induct
ion of inducible nitric oxide synthase (iNOS) after experimental SAH.
Methods. Chronic vasospasm was induced in male Wistar rats by an injection
of autologous blood (100 mu l) into the cisterna magna followed by a second
injection 24 hours later. A control group of 10 animals was treated with i
njections of 0.9% sodium chloride solution. Vasospasm was verified by press
ure-controlled angiography after retrograde cannulation of the external car
otid artery 7 days later. In 11 of 15 animals radiographic evidence of cere
bral vasospasm was seen. The animals were perfusion fixed and their brains
were removed for immunohistochemical assessment. With the aid of a microsco
pe, staining for iNOS was quantified in 40-mu m floating coronal sections.
Immunohistochemical staining for iNOS was markedly more intense in animals
with significant angiographic evidence of vasospasm. Virtually no staining
was observed in control animals. Seven days after the second experimental S
AH, labeling of iNOS was found in endothelial cells, in vascular smooth-mus
cle cells, and, above all, in adventitial cells. Some immunohistochemical s
taining of iNOS was observed in rod cells (activated microglia), in glial n
etworks, and in neurons.
Conclusions. The present study demonstrates induction of iNOS after experim
ental SAH.