Sirodesmin-deficient mutants were produced from aggressive isolates of Phom
a lingam by UV-mutagenesis. The mutation rates were 7.1 x 10(-3) for mutant
s expressing a 100-fold reduction of sirodesmin PL in liquid cultures and 1
.8 x 10(-3) for mutants with a 1000-fold reduction. The low ability of the
mutants to produce sirodesmins was also apparent in planta after infection
of cotyledons or the stem basis of Brassica napus. Growth rates under in vi
tro conditions, colony morphology, mating types and the formation of pycnid
ia and asci were not affected by the mutation. The mutants had the same abi
lity as the wild types to infect cotyledons of the susceptible B. napus cv.
Lirabon and to cause a typical grey-green tissue collapse. On the stem bas
is of the cv. Cobra, however, mutants caused significantly smaller lesions.
These results indicate that phytotoxic sirodesmins produced by P. lingam h
ave no basic role in pathogenesis and apparently are not involved in the vi
rulence on cotyledons. However, it cannot be excluded that the compounds ac
t as virulence factors on the stem base of B. napus.