The present work shows a methodology for the application of EDXRF with a X-
ray tube, in the analysis of elements in the following matrices: microalgae
, marine algae, marine sediments and corals. Various methods were applied:
one using a relative external standard, a fundamental parameters method, an
absolute method based on elemental sensitivity, a method which used X-ray
fluorescence excitation measured in the back side of the sample for low con
tents heavy elements determination and a TRXRF method. The thin layer sampl
es were prepared using a plastic resin. The analyzed samples of marine alga
e, marine sediments and corals belong to a marine ecosystem from the North
of Havana City, the most affected area by natural metereological phenomena
and human impact.