A kit of human polyclonal IgG for the diagnosis of infectious processes

The aim of this work was to obtain a freeze-dried kit for direct Tc-99m-lab eling of human polyclonal IgG. The labeling procedure was carried out by Sc hwarz's method. The best yields of Tc-99m-IgG were obtained by using sodium pyrophosphate decahydrate as a weak chelating agent. Performed tests showe d the stability of the radiopharmaceutical up to 24 hours. Plasma clearance in rats was fitted to a biexponential curve with T-1/2 alpha = (0.1+/-0.9) h and T-1/2 beta = (10+/-3)h. The organs with higher uptake of radiopharma ceutical were lung, kidneys and blood. In a rabbit model the abscess target /background ratio was 3-6 according to time of the scintigraphic images. Th irty patients with musculoskeletal infection were studied. Twenty-one lesio ns were detected and confirmed by culture/biopsy.