Lifetime measurement in Positron Annihilation Spectroscopy (PAS) is applied
to the study of free-volume collagen characteristics as a function of conc
entration. The lifetimes of positrons were measured by a conventional fast-
fast coincidence system. All lifetime data are fitted in three components b
y using the computer program POSITRON-FIT and resolved. For each concentrat
ion, lifetime distributions were analyzed in order to obtain the different
components, thus we have observed three components of which one long compon
ent tau(3). This long lived component can be associated with a pick-off ann
ihilation of ortho-positronium (o-Ps) trapped in free volumes of amorphous
region. This investigation shows the potential of positron annihilation spe
ctroscopy in the study of biopolymer microstructures.