Identification of domestic rice cultivars by RAPD method using milled ricegrains as samples

As it is obliged that cultivars, location and year of production should be displayed on bag of milled rice grains, technology to identify them by the objective methods is indispensable. Milled rice grains produced at the seve ral agricultural experiment stations were subjected to the RAPD method. Eac h DNA was extracted by CTAB method and amplified by PCR method using 10 mer primers. It became possible to identify the rice cultivars using milled ri ce grains as samples by the electrophoresis of the PCR products among the d ominant 10 rice cultivars in Japan.