Bacterial surface display of an anti-pollutant antibody fragment

A peptidoglycan-associated lipoprotein (PAL) fused to an antibody fragment (scFv) specific to the herbicide and environmental pollutant atrazine, has been successfully targeted to the cell surface of Escherichia toll. Anti-at razine binding could be observed via an atrazine-alkaline phosphatase conju gate. Cells containing the PAL fusion grew with little cellular toxicity wh en compared with the control. In contrast, expression of anti-atrazine anti body fragments alone caused the cells to lyse after 4 h. The surface displa y of anti-pollutant antibodies may have a future role in the bioremediation of contaminated water or the development of pollutant-specific, whole-cell biosensors.