Construction and characterization of a sheep BAC library of three genome equivalents

Authors
Vaiman, D Billault, A Tabet-Aoul, K Schibler, L Vilette, D Oustry-Vaiman, A Soravito, C Cribiu, EP
Citation
D. Vaiman et al., Construction and characterization of a sheep BAC library of three genome equivalents, MAMM GENOME, 10(6), 1999, pp. 585-587
Citations number
21
Categorie Soggetti
Molecular Biology & Genetics
Journal title
MAMMALIAN GENOME
ISSN journal
09388990 → ACNP
Volume
10
Issue
6
Year of publication
1999
Pages
585 - 587
Database
ISI
SICI code
0938-8990(199906)10:6<585:CACOAS>2.0.ZU;2-D
Abstract
A sheep BAC library of over three genome equivalents was constructed and ar rayed in superpools and row, column, and plate pools. The library contains 90,000 clones distributed in 39 superpools. The average insert size was est imated at 123 kb. The library was screened by PCR with 77 primer pairs corr esponding to ovine microsatellites distributed throughout the genome. The p robability of finding a random sequence in the library could be estimated a t 0.96.