Identification of bacteria using two degenerate 16S rDNA sequencing primers

Authors
Boye, K Hogdall, E Borre, M
Citation
K. Boye et al., Identification of bacteria using two degenerate 16S rDNA sequencing primers, MICROBI RES, 154(1), 1999, pp. 23-26
Citations number
14
Categorie Soggetti
Biology
Journal title
MICROBIOLOGICAL RESEARCH
ISSN journal
09445013 → ACNP
Volume
154
Issue
1
Year of publication
1999
Pages
23 - 26
Database
ISI
SICI code
0944-5013(199905)154:1<23:IOBUTD>2.0.ZU;2-Y
Abstract
Two degenerate 16S rDNA primers have been designed for broad-range identifi cation of eubacteria by PCR and automated sequencing. Using a simple method , the primers have proven useful in identification of proteobacteria (Campy lobacter Enterobacter; Escherichia, Helicobacter, Klebsiella), gram positiv e bacteria (Mycobacterium, Staphylococcus, Streptococcus) and spirochetes ( Borrelia) derived from clinical samples. In several cases, the samples coul d be identified at the species level.