L-deprenyl induces aromatic L-amino acid decarboxylase (AADC) mRNA in the rat substantia nigra and ventral tegmentum - An in situ hybridization study

L-Deprenyl is a complex drug, and number of mechanisms have been proposed t o explain its effects. These include blockade of dopamine metabolism, ampli fication of dopamine responses, induction of superoxide dismutase or delayi ng apoptosis. Using in situ hybridization techniques, we have shown that L- deprenyl (5-10 mg/kg intraperitoneally, killed after 24 h) increases aromat ic L-amino acid decarboxylase (AADC) mRNA levels in rat substantia nigraven tral tegmental area. In human brain tissue, AADC is present at low levels, suggesting a possible rate-limiting role in monoamine synthesis. This is pa rticularly important in parkinsonian patients, since the therapeutic effica cy of L-DOPA is attributed to its enzymatic decarboxylation to dopamine. Th e present findings support that one of the effects of L-deprenyl may be to facilitate the decarboxylation of L-DOPA by increasing the availability of AADC.