CD44 expression in cervical intraepithelial neoplasia (CIN) and carcinoma

Citation
H. Gorham et al., CD44 expression in cervical intraepithelial neoplasia (CIN) and carcinoma, MOL DIAGN, 4(1), 1999, pp. 45-56
Citations number
29
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology
Journal title
MOLECULAR DIAGNOSIS
ISSN journal
10848592 → ACNP
Volume
4
Issue
1
Year of publication
1999
Pages
45 - 56
Database
ISI
SICI code
1084-8592(199903)4:1<45:CEICIN>2.0.ZU;2-F
Abstract
Background: The expression of the CDU gene is markedly changed in many neop lastic tissues. The identification of tumor-specific CD44 expression patter ns may aid tumor diagnosis. Methods and Results: The transcription and translation of the CD44 gene wer e analyzed by reverse transcription polymerase chain reaction (RT-PCR), ill situ hybridization and by immunohistochemistry. Samples were obtained from 24 normal and 24 neoplastic or malignant human cervical tissues. Southern blot hybridization analysis of RT-PCR products revealed an increase in the size and number of CD44 standard and variant transcripts in malignant cervi cal tissues compared with corresponding normal tissues. Misprocessing of mR NA was indicated in cervical carcinoma cells by the retention of intronic s equences Multiple CD44 mRNA and protein isoforms were present throughout ca rcinoma tissues, whereas localization was restricted to the basal epitheliu m in normal cervical tissue. Analysis of desquamated cervical cells from ca ses of cervical intraepithelial neoplasia stages I-III showed progressively deranged patterns of CD44 expression, with more alterations being detected in the more advanced stages. Conclusions: Marked alterations in CDW expression occur in cervical tissues during progression to malignancy. CD44 expression analysis could aid the e arly diagnosis of cervical malignancy using minimally invasive methods.