Calcitonin gene-related peptide receptors in pig spleen and the involvement of the CGRP(1) receptor in the splenocyte function

Citation
H. Haberstock-debic et al., Calcitonin gene-related peptide receptors in pig spleen and the involvement of the CGRP(1) receptor in the splenocyte function, NEUROPEPTID, 33(1), 1999, pp. 47-53
Citations number
23
Categorie Soggetti
Neurosciences & Behavoir
Journal title
NEUROPEPTIDES
ISSN journal
01434179 → ACNP
Volume
33
Issue
1
Year of publication
1999
Pages
47 - 53
Database
ISI
SICI code
0143-4179(199902)33:1<47:CGPRIP>2.0.ZU;2-X
Abstract
Calcitonin gene-related peptide (CGRP)-positive nerve fibers have been foun d in the trabecula and parenchyme area of pig spleen. Receptor studies have demonstrated that the CGRP binding site in pig spleen membranes has an ave rage K-d 2.24 +/- 0.48 nM and B-max 78 +/- 4.09 fmol/mg of protein. In the K-d range demonstrated in the binding studies, the dose-dependent suppressi ve effect of CGRP on spleen T lymphocyte proliferation was found with the m aximal effect in 10(-9) M concentration. The same effect, but in a differen t concentration, was found on peripheral blood T lymphocytes with the maxim um in 10(-6) M concentration. Contrary to the results obtained through the simultaneous presence of CGRP and mitogen, preincubation with CGRP led to a stimulation of peripheral blood lymphocytes in response to ConA and had no effect on spleen T lymphocytes. These results illustrate the difference in CGRP effect between lymphocytes of different origins. Using CGRP, receptor antagonist CGRP(8-37), we established that the CGRP suppressive effect on spleen T lymphocyte proliferation is CGRP(1)-receptor mediated.