Inhibitory effects of cytokines on ovarian and endometrial carcinoma cellsin vitro with special reference to induction of specific transcriptional regulators
M. Seppanen et al., Inhibitory effects of cytokines on ovarian and endometrial carcinoma cellsin vitro with special reference to induction of specific transcriptional regulators, ONCOL RES, 10(11-12), 1998, pp. 575-589
In present study, we have investigated the effects of interferons-alpha (IF
N-alpha) and -gamma (IFN-gamma), interleukin-10 (IL-10) and -13 (IL-13), tr
ansforming growth factor-beta 1 (TGF-beta(1)), granulocyte-macrophage colon
y-stimulating factor (GM-CSF), and tumor necrosis factor-alpha (TNF-alpha)
on cell proliferation and induction of transcription factors AP-1 and NF-ka
ppa B in UM-EC-3 human endometrial adenocarcinoma cells and UT-OC-5 ovarian
carcinoma cells in vitro. In addition, cellular DNA was extracted to study
if any of these factors is able to induce apoptosis. In UM-EC-3 cell line
DNA synthesis was inhibited by GM-CSF, IL-10, IL-13, TGF-beta(1), IFN-alpha
, and IFN-gamma after 48 and 72 h in culture, whereas TNF-alpha had no sign
ificant effect on cell proliferation in any of the experiments. The inhibit
ion of DNA synthesis was similarly observed in UT-OC-5 ovarian carcinoma ce
lls by IL-10, TNF-alpha, and IFN-gamma after 48 and 72 h, whereas IFN-alpha
bad no statistically significant effect. An inhibitory effect of GM-CSF wa
s observed only after 48 h and TGF-beta after 72 h in culture, respectively
. Transcription factors AP-1 and NF-kappa B were both constitutively active
in UM-EC-3 and UT-OC-5 cells. The binding activity of AP-1 was found to be
stimulated by all growth-inhibitory cytokines studied in both cell lines,
whereas the specific binding activity of NF-kappa B was affected moderately
only by TNF-alpha in UT-OC-5 ovarian carcinoma cells. No signs of DNA frag
mentation typical of apoptosis were observed in any of these studies.