Human calicivirus-associated sporadic gastroenteritis in Finnish children less than two years of age followed prospectively during a rotavirus vaccine trial
Xl. Pang et al., Human calicivirus-associated sporadic gastroenteritis in Finnish children less than two years of age followed prospectively during a rotavirus vaccine trial, PEDIAT INF, 18(5), 1999, pp. 420-426
Background. Human caliciviruses (HuCV) cause outbreaks of gastroenteritis,
but their role in sporadic diarrhea in young children is not well-establish
ed.
Methods. Children (n = 2398) participating in a trial of oral rhesus-human
reassortant rotavirus tetravalent (RRV-TV) vaccine were evaluated from 2 mo
nths to 2 years of age, Stool specimens from 1477 episodes of acute gastroe
nteritis (788 in a placebo and 689 in a RRV-TV vaccine recipient group) wer
e tested for human calicivirus (HuCV) by reverse transcriptase-PCR with the
use of broadly reactive primers, and positive results were confirmed by So
uthern hybridization with probes specific for main genetic clusters of Geno
groups I and II of HuCV,
Results. HuCV were detected in 158 (20%) and 155 (22%) cases of gastroenter
itis in the placebo and RRV-TV vaccine groups, respectively. According to h
ybridization results, 8% of HuCV were of Genogroup I and 92% were of Genogr
oup II. The peak season of HuCV gastroenteritis was from November to Februa
ry. Of the 148 patients with pure HuCV infection in the placebo group, 89%
had vomiting, 79% had watery diarrhea, 21% had fever, 28% needed oral rehyd
ration and 1.4% were hospitalized. The diarrhea in HuCV gastro enteritis wa
s much less severe than that in rotavirus gastroenteritis, but vomiting was
equally severe. There was no effect of RRV-TV vaccine on the frequency or
clinical severity of HuCV gastroenteritis.
Conclusion. HuCVs are second in frequency to rotaviruses as causative agent
s in acute gastroenteritis in young children in the community.