Maize genes specifically expressed in the outermost cells of root cap

Citation
T. Matsuyama et al., Maize genes specifically expressed in the outermost cells of root cap, PLANT CEL P, 40(5), 1999, pp. 469-476
Citations number
21
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT AND CELL PHYSIOLOGY
ISSN journal
00320781 → ACNP
Volume
40
Issue
5
Year of publication
1999
Pages
469 - 476
Database
ISI
SICI code
0032-0781(199905)40:5<469:MGSEIT>2.0.ZU;2-M
Abstract
The cells at the periphery of the root cap are continuously sloughed off fr om the root into the mucilage, and are thought to be programmed to die. By using subtractive hybridization/differential screening and a transmembrane- domain trapping screening strategy involving expression screening in transf ected COS-7 cells, we isolated two related maize cDNAs (ZmRCP1 and ZmRCP2) that are specifically expressed in the outermost one to three cells of the cap. ZmRCP1 and ZmRCP2 are homologous proteins of 37 kDa mature polypeptide s with a region of regularly-spaced Cys residues and putative N-terminal si gnal peptides, and represent members of a novel protein family which is con served among angiosperm and gymnosperm.